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Figure 1 | Critical Care

Figure 1

From: Platelet-derived exosomes induce endothelial cell apoptosis through peroxynitrite generation: experimental evidence for a novel mechanism of septic vascular dysfunction

Figure 1

Tetraspan protein enrichment characterizes exosomes. The graph shows the percentage of positive events per 100,000 counts as analyzed by flow cytometry. Values are corrected for background and non-specific antibody binding. Exosomes obtained from septic patients as well as from platelets activated by the nitric oxide donor diethylamine-NONOate (NONOate; 0.5 μM) or lipopolysaccharide (LPS; 100 ng/ml) expose larger amounts of tetraspan protein family members CD9, CD63, and CD81, and less phosphatidylserine (as assessed by annexin V staining) than particles obtained from platelets treated only with saline (Control) or thrombin (5 IU/ml) or from apoptotic endothelial cells (apoptosis). Results are means ± SD. For each bar, n = 4 samples. *P < 0.05 versus control, †P < 0.05 versus apoptotic bodies (apoptosis). UV, ultraviolet.

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