Figure 7

Effects of hyperoxia effects on stretch-induced endothelial nitric oxide synthase activation of airway epithelium. Representative photomicrographs (×400) with phosphorylated endothelial nitric oxide synthase staining of the lung sections after 5 hours of mechanical ventilation with room air or hyperoxia (n = 6/group). (a) Control wild-type mice with room air. (b) Control wild-type mice with hyperoxia. (c) Tidal volume 30 ml/kg wild-type mice with room air. (d) Tidal volume 30 ml/kg wild-type mice with hyperoxia. (e) Tidal volume 30 ml/kg Akt^+/- mice with room air. (f) Tidal volume 30 ml/kg Akt^+/- mice with hyperoxia. A dark-brown diaminobenzidine signal indicates positive staining of lung epithelium, while lighter shades of bluish tan signify nonreactive cells. Akt, serine/threonine kinase/protein kinase B.