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Figure 8 | Critical Care

Figure 8

From: Hyperoxia increases ventilator-induced lung injury via mitogen-activated protein kinases: a prospective, controlled animal experiment

Figure 8

Apoptosis of airway epithelium during high-tidal volume (VT) ventilation with or without hyperoxia. Representative photomicrographs (×400) with TUNEL staining (a-c) (n = 5 per group) and electron micrographs (d-f) (n = 2 per group) of the lung sections after five hours of high-VT (30 ml/kg) mechanical ventilation without or with hyperoxia. (a,d) Control non-ventilated wild-type mice. (b,e) Wild-type mice ventilated at a VT of 30 ml/kg with room air. (c,f) Wild-type mice ventilated at a VT of 30 ml/kg with hyperoxia. Apoptotic cells are identified by asterisks or arrows. A dark brown DAB signal indicates positive staining of apoptotic cells, and shades of blue-green to greenish tan signify non-reactive cells. Highly condensed and fragmented heterochromatin of bronchial epithelial cells indicates apoptosis. Scale bars = 20 μm (a-c), 4 μm (d-f). DAB, diaminobenzidine; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling.

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