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Figure 6 | Critical Care

Figure 6

From: The early responses of VEGF and its receptors during acute lung injury: implication of VEGF in alveolar epithelial cell survival

Figure 6

Lung epithelial cell death induced by vascular endothelial growth factor (VEGF) or vascular endothelial growth factor receptor (VEGFR)-1 knock-down. Human A549 cells were cultured in 24-well plates and transfected with 50 nM of small interference RNA (siRNA) specifically against either VEGF or VEGFR-1, or with a non-specific duplex RNA (NS) as control for 24 h. (a) VEGF or VEGFR-1 siRNA induced changes in cell number and/or cell morphology in A549 cells. Pictures shown are representatives for each treatment (magnification 400×). (b) XTT assay for cell viability. The data shown are mean ± standard deviation from three separated experiments. **p < 0.01 versus the non-specific control. (c) VEGF and VEGFR-1 immunofluorescent staining at 24 h after treatment with different RNA duplexes. Decreased staining was noted in siRNA treated cells. (d) Western blots confirmed that siRNA specifically reduced VEGF or VEGFR-1 protein expression.

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Critical Care

ISSN: 1364-8535