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Figure 4 | Critical Care

Figure 4

From: The early responses of VEGF and its receptors during acute lung injury: implication of VEGF in alveolar epithelial cell survival

Figure 4

Intestinal ischemia reperfusion (IIR)-induced changes of vascular endothelial growth factor receptor (VEGFR)-2 in the lung tissue and immunoblotting of VEGF and its receptors. (a) VEGFR-2 immunostaining (four animals per group). Slides (magnification 1,000×) shown are representatives of indicated groups. Positively stained cells are in brown (examples are indicated with arrowheads). In the IIR group, some of the positive cells appear to be interstitial monocytes with strong staining in the cytoplasm. (b) Quantification of VEGFR-2-positive cells per field. Ten fields were counted from each animal and four animals from each group. (c) Western blotting for VEGF and its receptors. Results from two animals per group are used as examples. The optical density of blot bands were quantified with desitometry and normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as controls. No significant difference was found among these three groups.

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Critical Care

ISSN: 1364-8535