Figure 1

Parameters of apoptosis. (a) TdT-mediated dUTP nick end labeling (TUNEL)-positive cells (upper panel), changes in caspase 3 activity (middle panel), and changes in activated caspase 3 expression as analyzed by Western blotting (lower panel) in the left lower lobe of the different groups. Values are expressed as means ± standard error of the mean (n = 5–6 cats/group). (b) Representative Western blot analysis of caspase 3. The groups were as follows: i = nonischemic group; ii = ischemia/reperfusion; iii, iv and v = IB-MECA 50 μg/kg, 100 μg/kg and 300 μg/kg, respectively, administered 1 hour before reperfusion; vi and vii = MRS3558 50 μg/kg and 100 μg/kg, respectively, administered before reperfusion; and viii = MRS1191 pretreatment before MRS3558 (100 μg/kg) and beginning of reperfusion. *P < 0.05 versus the other groups (i, ii, iii, and viii).