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Figure 2 | Critical Care

Figure 2

From: The use of moderate hypothermia during cardiac surgery is associated with repression of tumour necrosis factor-α via inhibition of activating protein-1: an experimental study

Figure 2

DNA binding activity of NF-κB: hypothermia versus normothermia. (a) DNA binding activity of NF-κB, as measured by EMSA and confirmed by supershift in myocardium, in pigs operated on under moderate hypothermia (28°C; circles) or normothermia (37°C; squares). The time points of evaluation were as follows: 1 = before CPB; 2 = before aortic cross clamping; 3 = before removal of aortic clamp; and 4 = 6 hours after CPB. Data are expressed as mean ± standard deviation. (b) Example gel showing the effect of temperature during CPB on activation of NF-κB as detected by EMSA and confirmed by supershift with anti-p50 and anti-p65 in pigs operated on under hypothermia (28°C) or normothermia (37°C). Results are representative of four independent experiments in each group. CNT, counts/mm2; CPB, cardiopulmonary bypass; EMSA, electrophoretic mobility shift assay; NF-κB, nuclear factor-κB; NS, nonspecific.

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