Figure 1

The approach to identify mechanical stress induced candidate genes using schematic representation of cross-species ortholog database and gene ontology processes. Total RNA from rat, mouse, and canine ventilator-injured lung tissues and human endothelial cell cultures exposed to injurious mechanical stretch was extracted, and gene expression data were generated by hybridization of corresponding total mRNAs to HG-U34A, HG-U74A, HG-U133A, and HG-U95A Affymetrix arrays, respectively. These steps were performed at the PGA-HopGene center. Gene expression profiles were analyzed by the HopGene PGA center using in silico multispecies cross-platform database applying the RESOURCERER tool designed by the TIGR PGA center. Gene ontology assignment and analysis was conducted by HopGene PGA center using the MAPPFinder tool designed by the BayGenomics PGA center. The acute lung injury (ALI)-related ontologic groups were selected using filtering conditions described in Table 1. The contribution of each bioprocess to ALI is represented as the percentage of values calculated by MAPPFinder and shown in the percentage changed genes column. The literature search was done by the HopGene PGA center using PubMatrix tool designed by PGA collaborators at the National Institute on Aging, National Institutes of Health.