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Figure 3 | Critical Care

Figure 3

From: Insulin-like growth factor-1 protects ischemic murine myocardium from ischemia/reperfusion associated injury

Figure 3

Measured creatine phosphokinase in perfusate from reperfused murine hearts. Hearts were prepared for Langendorf ex vivo reperfusion and perfused until the monitored heart rate and pressure were stable (approximately 3–5 min); then they were subjected to 20 min of ischemia followed by reperfusion. Perfusate solution was collected (approximately 1 ml/4 min) around each time point for determination of CPK activity. For all time points, tumour necrosis factor (TNF)-α reperfusion generated a significant elevation in the detectable amount of CPK activity relative to that detected with insulin-like growth factor (IGF)-1 reperfusion (P < 0.005, by analysis of variance).

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