Figure 1
From: Neutrophil 'connectivity': key to neutrophil-mediated tissue injury?
Confocal laser scanning microscopy images of immunostained peripheral blood neutrophils of asthmatic subjects. Representative images of human neutrophils stained with Alexa-conjugated secondary antibody (red) to detect elastase, and BODIPY-FL-conjugated secondary antibody (green) to detect immunoreactivity against CD63. (a) Resting peripheral blood neutrophil labeled with Alexa indicating elastase immunoreactivity. (b) Resting peripheral blood neutrophil labeled with BODIPY-FL indicating CD63 immunoreactivity. (c) Combined image of CD63 and elastase immuno-staining in resting cells. (d) IL-8-activated (50 ng/ml) peripheral blood neutrophil labeled with Alexa indicating elastase immunoreactivity. (e) IL-8-activated (50 ng/ml) peripheral blood neutrophil labeled with BODIPY-FL indicating CD63 immunoreactivity. (f) Combined image of CD63 and elastase immunostaining in IL-8-activated (50 ng/ml) peripheral blood neutrophil. Original magnification, 63 × 10 for all images.