Effects of splenectomy and GTS-21, a selective α7 nicotinic acetylcholine receptor agonist, on the development of septic ileus in mice

Table 1 %GE, GC, serum cytokine levels (pg/ml) and colonic cytokine levels (pg/g colon) in mice that underwent splenectomy or sham surgery, followed by the CLP procedure and administration of vehicle or 8 mg/kg GTS-21

GI transit study (n= 11)	%GE	GC
Nonseptic animals	88.96 ± 8.13	4.86 ± 0.45
Sham + vehicle + CLP	53.27 ± 10.32	2.53 ± 0.42
Sham + GTS-21 8 mg/kg + CLP	83.62 ± 5.91*	4.26 ± 0.35*
SPLX + vehicle + CLP	79.49 ± 8.98*	3.86 ± 0.44*
SPLX + GTS-21 8 mg/kg + CLP	77.64 ± 11.81*	3.55 ± 0.52
Cytokine level in serum ( n = 8 to 11)	IL-6	TNFα
Nonseptic animals	2.57 ± 0.48	4.95 ± 0.29
Sham + vehicle + CLP	243.56 ± 54.89	63.72 ± 21.25
Sham + GTS-21 8 mg/kg + CLP	90.26 ± 31.31*	54.23 ± 25.14
SPLX + vehicle + CLP	74.31 ± 17.45*	11.52 ± 1.99^#
SPLX + GTS-21 8 mg/kg + CLP	64.98 ± 7.15*	12.81 ± 3.91^#
Cytokine levels in colonic tissue ( n = 5 to 11)	IL-6	TNFα
Nonseptic animals	20.43 ± 4.87	41.30 ± 4.47
Sham + vehicle + CLP	1281.85 ± 932.10	137.15 ± 61.36
Sham + GTS-21 8 mg/kg + CLP	134.88 ± 36.15	46.46 ± 5.03*
SPLX + vehicle + CLP	214.22 ± 75.88	29.97 ± 8.60*
SPLX + GTS-21 8 mg/kg + CLP	487.49 ± 343.87	46.03 ± 17.22*

Data presented as mean ± SEM. Two-way ANOVA or one-way ANOVA as appropriate with SNK post hoc testing; *P ≤ 0.05 versus sham + vehicle, ^#P ≤ 0.05 significant effect of splenectomy (SPLX).

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