Figure 1

Phenotypic characterisation of interphase neutrophils in sepsis patients. (A) Flow cytometric evaluation of interphase cells from a representative healthy control and interphase cells and PMN from a representative septic shock patient. Gated regions illustrate: (i) monocytes, (ii) interphase neutrophils and (iii) PMN. The proportion of interphase neutrophils was determined using flow cytometry by gating region ii, and expressed as a percentage of all viable cells. (B) The proportion of interphase neutrophils collected following gradient separation of blood from patients with septic shock (n = 12), sepsis without shock (n = 12) and hospital controls (n = 12) on day 0 (enrolment) and day 2. Horizontal lines show the median and interquartile range. (C) Microscopic identification of interphase cells in longitudinal samples from a representative septic shock patient. (D) Fluorescence-activated cell sorting (FACS) Calibur flow cytometric phenotyping of (i) monocytes, (ii) interphase neutrophils and (iii) PMN from a representative septic shock patient. The first peak represents background or negative staining. (E) Gallios median fluorescence intensity (MFI) of CD15 expressed on paired non-cryopreserved interphase neutrophil and polymorphonuclear neutrophils (PMN) from four septic shock patients (three with a longitudinal day-2 or day-3 sample). P-values were derived using a non-parametric analysis of the seven paired interphase neutrophil and PMN data points.