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Table 4 Results of immunochemical measurements in alveolar type II cells a

From: Double impact of cigarette smoke and mechanical ventilation on the alveolar epithelial type II cell

Cell type NSNV SNV NSV SV
Catalase, nmol/min (n = 8 for each group) 3.40 × 10-5 ± 2.52 × 10-5 8.01 × 10-6 ± 9.03 × 10-6 8.93 × 10-5 ± 1.19 × 10-4 2.54-5 ± 5.72-5
Toll-like receptor 4, μg/cell (n = 8 for each group) 3.82 × 10-7 ± 2.92 × 10-7 1.81 × 10-7 ± 1.28 × 10-7 6.94 × 10-7 ± 5.17 × 10-7 1.07 × 10-6 ± 8.14 × 10-7
NFκBp65, ng/cell (n = 8 for each group) 4.20 × 10-6 ± 3.43 × 10-6 4.14 × 10-6 ± 1.89 × 10-6 5.08 × 10-6 ± 4.06 × 10-6 9.28 × 10-6 ± 7.25 × 10-6
HSP70, ng/cell (n = 8 for each group) 1.75 × 10-7 ± 1.05 × 10-7 5.24 × 10-7 ± 2.27 × 10-7 6.37 × 10-7 ± 5.23 × 10-7 1.24 × 10-6 ± 1.11 × 10-6
Caspase 3, U/cell (n = 10 for each group) 3.56 × 10-4 ± 3.22 × 10-4 4.14 × 10-4 ± 2.48 × 10-4 1.72 × 10-4 ± 1.11 × 10-4 1.01 × 10-3 ± 1.13 × 10-3
Caspase 8, U/cell (n = 7 for each group) 5.13 × 10-8 ± 2.71 × 10-8 7.42 × 10-8 ± 4.71 × 10-8 3.85 × 10-8 ± 1.38 × 10-8 1.76 × 10-7 ± 1.11 × 10-7
Caspase 9, U/cell (n = 7 for each group) 6.03 × 10-8 ± 3.71 × 10-8 1.65 × 10-7 ± 1.05 × 10-7 4.70 × 10-8 ± 1.39 × 10-8* 3.51 × 10-7 ± 4.20 × 10-7
DNA fragments, % absorbance compared with NSNV (n = 8 for each group) 100.00 ± 78.57 62.19 ± 108.49 15.73 ± 129.04 143.07 ± 69.43*
  1. aHSP: Heat shock protein; NSNV: Nonsmoke/nonventilated; NSV: Ventilation alone; NFκB: Nuclear factor κB; SV: Mechanical ventilation. *P < 0.05 vs. NSNV; Data are means ± SD.