Flow cytometric analysis of the frequency of HLA-DR + and Tim-3+ monocytes. Peripheral whole-blood samples were collected from 13 patients and 11 healthy controls, stained with the fluorescent antibodies against CD14 (anti-CD14) and major histocompatibility complex class II cell surface receptor encoded by the human leukocyte antigen (anti-HLA-DR) or T-cell immunoglobulin mucin 3 (anti-Tim-3), and measured for the percentage of HLA-DR + CD14+ or Tim-3 + CD14+ monocytes in the total CD14+ monocytes. Data are presented as flowcharts and expressed as the means of individual participants. The Mann–Whitney U test was used to analyze the differences between groups. (A) The gating strategy and representative flow cytometric plots of Tim-3+ and HLA-DR + monocytes are shown. FITC, fluorescein isothiocyanate; FSC Lin, forward scatter area; SSC Lin, side scatter area. (B) Quantitative analysis.