Figure 4From: Hypercapnia attenuates ventilator-induced diaphragm atrophy and modulates dysfunctionDiaphragm ubiquitinated myosin and representative blot, proteasome activity, LC3B-II content and representative blot. (a) Concentration of ubiquitinated myosin per total myosin in diaphragm of MV and MV + H groups showed an increased trend compared with control, but was not significantly different between groups. (b) Representative Western blot of ubiquitinated myosin. (c) 20S proteasome activity in diaphragm. Hypercapnic ventilated animals demonstrate decreased activity versus control animals (*P <0.05). (d) LC3B-II content. Both ventilated and hypercapnic ventilated animals were significantly different compared with control (*P <0.05). (e) Representative Western blot against LC3B-II. LC3B-II, light chain 3B-II; MV, mechanically ventilated; MV + H, mechanically ventilated with hypercapnia.Back to article page