Figure 3

Receptor for advanced glycation end products ( Rage ) ^−/−but not Toll-like receptor (tlr ) 4^−/−mice show reduced lung pathology during S. aureus pneumonia. Representative slides of lung HE staining of wild-type (Wt) (A), tlr4^−/−(C) and rage^−/− mice (E), original magnification × 2. The boxed areas are also shown at a higher magnification for Wt (B) tlr4^−/−(D) and rage^−/− mice (F), original magnification × 10. Scale bars indicate 200 μm. Total pathology scores at indicated time points after intranasal infection with S. aureus in Wt (dark gray), tlr4^−/− (light gray) and rage^−/− mice (white) were determined according to the scoring system described in the Methods section (G). Total protein was measured in bronchoalveolar lavage (BAL) fluid from Wt (dark gray), tlr4^−/− (light gray) and rage^−/− mice (white) at the indicated time points after intranasal S. aureus infection (H). Data are expressed as box-and-whisker diagrams depicting the smallest observation, lower quartile, median, upper quartile and largest observation (7 to 8 mice per group at each time point). ^*P <0.05, ^**P <0.01 versus Wt mice at the same time point.