Table 1 Primer designs
Gene | Accession no. | Sequence |
|---|---|---|
HLA-DRA | NM_019111 | 5′-GATGCTCCAAGCCCTCTCCCAG-3′ (probe) (22) |
5′-GCCTCTTCTCAAGCACTGGGA-3′ (sense) (21) | ||
5′-CCACCAGACCCACAGTCAGG-3′ (antisense) (20) | ||
HLA-DMA | NM_006120 | 5′-TCCCTGAAGCTCCTACTCCAA-3′ (probe) (22) |
5′-CTGTGTGGCAAGAAGGTATG-3′ (sense) (20) | ||
5′-TCCTGGCAGTACACTGTGT-3′ (antisense) (19) | ||
HLA-DMB | NM_002118 | 5′-GAGCAGGTGGCTTCGTGGC-3′ (probe) (19) |
5′-CATCTTTACAGAGCAGAGCAT-3′ (sense) (21) | ||
5′-ATGTGAAATCCTTTGGAGTCC-3′ (antisense) (21) | ||
CIITA | NM_000246 | 5′-CTCAGAACCCGACACAGACAC-3′ (probe) (21) |
5′-CCTGGCTGGAGAAGAAGAG-3′ (sense) (19) | ||
5′-TCCTGGAAGACATACTGGTC-3′ (antisense) (20) | ||
CD74 | NM_004355 | 5′-CCAGCGAGGAGCAGAGTCAC-3′ (probe) (20) |
5′-TTATCTCCAACAATGAGCAACT-3′ (sense) (22) | ||
5′-ACAGGAAGTAGGCGGTGGT-3′ (antisense) (19) | ||
HPRT1 | NM_000194.2 | 5′-CAAGTTTGTTGTAGGATATGCCC-3′ (probe) (23) |
5′-CCAAAGATGGTCAAGGTCGC-3′ (sense) (20) | ||
5′-GACACAAACATGATTCAAATCC-3′ (antisense) (22) | ||
PPIB | NM_000942.4 | 5′-GGTGAGCATGGCCAACGCAGG-3′ (probe) (22) |
5′-GGAGATGGCACAGGAGGAAAGA-3′ (sense) (22) | ||
5′-GGGAGCCGTTGGTGTCTTTG-3′ (antisense) (20) |