Figure 3

Endothelial homeostasis (A) Immunofluorescence for glomerular endothelial nitric oxide synthase (eNOS) at 24 hours post intervention and immunocytochemistry (ICC) for dolichos biflorus agglutinin (DBA) lectin. eNOS staining was almost completely absent in high-fat diet (HF) swine. Glomerular eNOS staining in cardiopulmonary bypass (CPB) swine was reduced and this was associated with endothelial dysfunction and reduced glomerular filtration rate (GFR). DBA lectin staining was reduced in CPB pigs indicative of glycocalyceal loss but was preserved in CPB + HF pigs indicating maintenance of endothelial homeostasis. There was increased microvascular staining density in HF pigs that may underlie the preservation of renal blood flow observed in CPB + HF pigs. (B) Quantification of ICC for DBA lectin staining expressed as percentage staining at 24 hours post injury. (C) Renal blood flow/kilogram body weight (RBF) measured on a single renal artery via laparotomy. NO dependent vascular reactivity was determined by the change in renal blood flow in response to an infusion of acetylcholine (RBF post Ach). (D) ATP and (E) adenosine levels, expressed as ratios in the renal cortex and medulla at 24 hours post intervention. Values represent mean (standard error of the mean) of at least n = 4 per group. *P <0.05 versus sham, †P <0.05 versus Sham + HF, ‡P <0.05 versus CPB.