Phosphorylation of cardiac Troponin I (cTnI). Western blot analysis were performed on myocardial biopsies of normothermic controls (separate series) and after rewarming from DHCA. Epinephrine determined a significant phosphorylation of cTnI in both conditions. Rewarming from DHCA produced a similar phosphorylation. Levosimendan did not cause phosphorylation of cTnI during normothermia and prevented the phosphorylation after rewarming from DHCA. Values determined by densitometry of phosphorylated-cTnI (p-cTnI) over the relative expression of total cTnI. Data are shown here as mean ± SEM (n = 5 per group). Symbol indicates intergroup statistical differences (*P<0.05). DHCA/REW, rewarming after deep hypothermic circulatory arrest.