Macrophage accumulation in the liver and lungs of septic mice. The upper panel displays liver parameters, the bottom panel displays lung parameters. Representative microscopic image of tissue macrophage staining (A, G). Computerized quantification of macrophage immunostaining (B, H). Gene expression of the M1-type macrophage marker tumor necrosis factor alpha (TNFα) (C, I), and M2-markers Arginase-1 (D, J) and CD163 (E, K). Gene expression of the chemo-attractant factor chemokine ligand 2 (CCL2) (F, L). AU is arbitrary units. Data boxes present median and IQR; whiskers represent the 10th and the 90th percentiles. White boxes present healthy controls (n = 13), light gray boxes are critically ill mice allocated to the total parenteral nutrition (TPN) protocol (cecal ligation and puncture (CLP)-fed) (n = 11), and dark gray boxes are critically ill mice allocated to the restriction protocol (CLP-restricted) (n = 11).