Figure 3
Adipogenic markers in subcutaneous and visceral adipose tissue of septic mice. The box plots represent median adipocyte size (A), estimated proportion of adipocytes and representative microscopic adipose tissue images of a healthy and a cecal ligation and puncture (CLP)-fed mouse (B), Proliferating Cell Nuclear Antigen (PCNA) gene expression (C), percentage stained area for peroxisome proliferator-activated receptor γ (PPARγ) (D), mRNA gene expression of CCAAT/enhancer binding protein-β (C/EBPβ) (E). AT is adipose tissue, A.U. is arbitrary units. Data boxes present median and IQR; whiskers represent the 10th and the 90th percentiles. For immunocytochemistry analyses in A, B and D, White boxes present healthy controls (n = 10), light gray boxes are critically ill mice allocated to the total parenteral nutrition (TPN) protocol (CLP-fed) (n = 6), and dark gray boxes are critically ill mice allocated to the nutrient restriction protocol (CLP-restricted) (n = 8). For gene expression analyses, the number of animals are healthy controls (n = 8), CLP-fed (n = 7), CLP-restricted (n = 6).