Figure 2

Co-localization of green fluorescent protein (GFP)-Microtubule-associated protein light chain 3 (LC3) dots with lysosome-associated membrane protein type 1 (LAMP1) in the liver after cecal ligation and puncture (CLP). (A) Confocal images of liver samples obtained from GFP-LC3 transgenic mice. Green-fluorescing GFP-LC3 dots were present in the cytosol. LAMP1 was stained using Cy3-conjugated IgG secondary antibodies. Merged images demonstrate co-localization of GFP-LC3 dots and LAMP1. (B) The number of GFP-LC3 or GFP-LC3 + LAMP1 dots per cellular confocal image was quantified at 6 and 24 h after CLP. All data are expressed as mean ± SD. Data were analyzed for statistical significance using the Mann-Whitney test. Differences in the number of GFP-LC3 or GFP-LC3 dots in CLP mice at 6 h compared with CLP mice at 24 h were statistically significant (^*P <0.05; n = 50 cells /animal; n = 4 animals). (C) Relative expression of p62 protein in the liver at 6 and 24 h after sham or CLP operation. The amount of p62 protein was normalized to that of β-tubulin by evaluation of band intensity from western blotting. All data were expressed as the mean ± SD. Data were analyzed for statistical significance using the Mann-Whitney test (^*P <0.05; n = 5 in each group).