Western blot analysis of protein expression. Western blots revealing (A) inducible nitric oxide synthase (iNOS), (B) p-AKT, (C) phosphorylated endothelial nitric oxide synthase (p-eNOS), (D) p-p38, (E) p-JNK and (F) p-ERK expression. Proteins are expressed in whole lysates of aorta (n = 8) from all groups of rats. A typical western blot is shown below each histogram. Densitometric analysis was used to calculate the normalised protein ratio (protein to α-tubulin), which was set at 1 for the control group. Data are expressed as mean ± standard deviation. *P <0.05, significantly different versus sham and all groups. **P <0.05 versus haemorrhagic shock (HS) + sodium hydrosulphide (NaHS) group. PAG, D,L-propargylglycine.