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Figure 5 | Critical Care

Figure 5

From: Effect of tyrosine kinase inhibitors, imatinib and nilotinib, in murine lipopolysaccharide-induced acute lung injury during neutropenia recovery

Figure 5

Effect of imatinib or nilotinib on activation of p-platelet-derived growth factor receptor-ß in mice with lipoplysaccharide-induced acute lung injury. Lung tissues were homogenized and the extracted protein samples were separated by SDS-PAGE using 8% PAGE gel, followed by transfer to 0.45 μm polyvinylidene fluoride membranes. The membranes were blocked with 5% skimmed milk and exposed overnight at 4°C to specific anti-platelet-derived growth factor receptor-ß (PDGFR-β) and anti-phospho-PDGFR-β antibodies. LPS, lipopolysaccharide; C, cyclophosphamide; I, imatinib; N, nilotinib.

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Critical Care

ISSN: 1364-8535