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Figure 5 | Critical Care

Figure 5

From: Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay

Figure 5

Effect of septic serum on dexamethasone inhibition of lipopolysaccharide-induced TNFα release in normal human peripheral blood mononuclear cells. The in vitro effect of septic serum on glucocorticoid sensitivity was evaluated in a dexamethasone cell sensitivity assay in control peripheral blood mononuclear cells in culture. Peripheral blood mononuclear cells from healthy control participants were cultured in the presence of pooled human septic serum taken from the patients on admission to the intensive care unit or pooled serum from the controls. After 48 h in culture, LPS or LPS plus dexamethasone 10-7 M was added to the medium. The TNFα released in response to LPS stimuli in the presence and absence of dexamethasone was analyzed by ELISA. The values are expressed as a percentage of TNFα released from cells cultured in the presence of LPS without dexamethasone. n = 5. CS, control serum; Dex, dexamethasone; LPS, lipopolysaccharide; SS, septic serum, TNFα, tumor necrosis factor alpha.

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Critical Care

ISSN: 1364-8535