Skip to main content
Figure 4 | Critical Care

Figure 4

From: Septic serum induces glucocorticoid resistance and modifies the expression of glucocorticoid isoforms receptors: a prospective cohort study and in vitro experimental assay

Figure 4

Effect of human septic serum on glucocorticoid receptor isoform expression in different cell lines. CEM, Raji and K562 cell lines were cultured in the presence of the patients' septic serum and serum obtained from the control group at 10% final concentration in the culture medium. After 48 h in culture, the GR protein expression was analyzed by western blotting with specific antibodies for GRα and β isoforms. The quantification of the western blot analysis of (A) GRα and (B) GRβ are shown (n = 9). The values were normalized to β actin expression and are expressed as percentage values of the control (GR cell expression cultured in the presence of control serum). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). Representative western blot analysis of GRα, β and β actin are shown for each cell line. CS, control serum; GR, glucocorticoid receptor; SS, septic serum.

Back to article page

Critical Care

ISSN: 1364-8535