Skip to main content
Figure 8 | Critical Care

Figure 8

From: Blockade ofthe negative co-stimulatory molecules PD-1 and CTLA-4 improves survival in primary and secondary fungal sepsis

Figure 8

Anti-PD-1 and anti-PD-L1 increase MHC II expression on dendritic and macrophages in fungal sepsis. Mice had CLP and three days later were injected with Candida.Anti-PD-1 or anti-PD-L1 was administered on days 5 and 8 post-CLP. Spleens were harvested on Day 9 post-CLP and splenocytes prepared.Immunostaining for dendritic cells (CD11c+/MCH II hi) and macrophages (F4/80+/MHC II intermediate) was performed.The mean fluorescence intensity (MFI) of dendritic cell and macrophage MHC II was decreased in fungal sepsis compared to naïve mice (P <0.05).Both anti-PD-1 and anti-PD-L1 increased MHC II MFI in dendritic cells and macrophages in fungal sepsis (P <0.05), but anti-PD-L1 had a greater effect compared to anti-PD-1, (P <0.05). CLP, cecal ligation and puncture; IFN-γ, interferon gamma; PD-1, programmed cell death 1; PD-L1, programmed cell death ligand 1.

Back to article page

Critical Care

ISSN: 1364-8535