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Figure 6 | Critical Care

Figure 6

From: Blockade ofthe negative co-stimulatory molecules PD-1 and CTLA-4 improves survival in primary and secondary fungal sepsis

Figure 6

Anti-PD-L1 increases IFN-γ production in two-hit fungal sepsis. Mice had CLP followed in three days by Candida injection.Anti-PD-1 and anti-PD-L1 were administered on days 5 and 8 post CLP and spleens harvested on Day 9.Splenocyte suspensions were prepared and stimulated overnight with anti-CD3/CD28.Supernatants were harvested at 18 h and IFN-γ production quantitated.Two-hit fungal sepsis caused a decrease in IFN-γ production compared to sham (P <0.05); splenocytes from mice treated with anti-PD-1 had an increased IFN-γ production compared to septic mice not receiving antibody therapy.There was a trend toward increased IFN-γ production in mice treated with anti-PD-L1 but it did not reach statistical significance. CD, cluster of differentiation; IFN-γ, interferon gamma; IL, interleukin; PD-1, programmed cell death 1; PD-L1, programmed cell death ligand 1.

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Critical Care

ISSN: 1364-8535