Skip to main content
Figure 5 | Critical Care

Figure 5

From: Blockade ofthe negative co-stimulatory molecules PD-1 and CTLA-4 improves survival in primary and secondary fungal sepsis

Figure 5

Anti-PD-1 increases IFN-γ+ CD4 and CD8 T cells in fungal sepsis. Mice had tail-vein injection of Candida and were treated with anti-CTLA-4 or anti-PD-1 as described in Figure 4. Following supernatant harvesting, splenocytes were treated with brefeldin for four hours and then fixed, permeabilized and immunostained for CD4, CD8 and intracellular IFN-γ.Flow cytometry was performed to quantitate the percentage of CD4 and CD8 T cells that were positive for IFN-γ.Anti-PD-1 but not anti-CTLA-4 increased the percentage of IFN-γ+ CD4 and CD8 T cells, (P <0.05). CD, cluster of differentiation; CTLA-4, cytotoxic T-lymphocyte antigen-4; IFN-γ, interferon gamma; PD-1, programmed cell death 1.

Back to article page

Critical Care

ISSN: 1364-8535