Figure 4

cRGD inhibits neutrophils and macrophages infiltrating the lungs after intestinal ischemia/reperfusion. Lung tissues harvested 4 hours after intestinal ischemia/reperfusion (I/R) were sectioned and immunostained with (A) Gr-1 (green) for detecting neutrophils and (B) CD11b (red, upper panel) for detecting macrophages. Lower panels: 4',6-diamidino-2-phenylindole (DAPI; blue) staining for nucleus. Representative images for sham, vehicle, and cRGD treatment groups at 200× magnification. Graphical representation of (C) Gr-1-positive and (D) CD11b-positive staining cells averaged over 10 microscopic fields per animal. Data expressed as mean ± standard error (n = 5/group). *P < 0.05 versus sham, ^#P < 0.05 versus vehicle. cRGD, cyclic arginine-glycine-aspartate peptide.