Figure 5
From: Mechanisms underlying ICU muscle wasting and effects of passive mechanical loading
Cross-sections of tibialis anterior muscle stained for neuronal nitric oxide synthase, Laminin and 4',6-diamidino-2-phenylindol. Cross-sections of tibialis anterior muscle stained for neuronal nitric oxide synthase (nNOS; red), Laminin (green) and 4',6-diamidino-2-phenylindol (DAPI; blue). In control, basal expression of nNOS is low and localized to the sarcolemma. ICU conditions induce strong expression of nNOS and dislocation of nNOS to the cytoplasm (arrowheads). Scale bar = 50 Ī¼m. The overlay image for each row is shown to the left.