Figure 4
From: Toll-like receptors expression and interferon-γ production by NK cells in human sepsis
Expression of CD69 on natural killer (NK) cell CD56bright and CD56dim subsets on freshly isolated cells or after in vitro cultures. (A) Representative flow cytometry histograms of surface basal expression of CD69 on CD56bright and CD56dim NK cell subsets for healthy volunteers, systemic inflammatory response syndrome (SIRS), and sepsis patients. Black line: isotype control; colored line: anti-CD69. (B) CD69 expression in all three groups for freshly isolated NK cells (basal) or after overnight culture in the presence of IL-15 + IL-18; data are shown as median and interquartile range. CD69 expression expressed after overnight culture in the presence of IL-15 + IL-18 and lipopolysaccharide (LPS) (C), CpG (D) and heat-killed S. aureus (HKSA) (E); data are expressed as median and interquartile range. *P < 0.05; **P < 0.01; ***P < 0.001 for comparison between healthy donors and patients. MFI, mean fluorescence intensity.