Figure 3
From: Toll-like receptors expression and interferon-γ production by NK cells in human sepsis
Flow cytometry analysis of Toll-like receptor 4 (TLR4) expression in natural killer (NK) cell CD56bright and CD56dim subsets. (A) Representative flow cytometry histogram of surface expression of TLR4 on CD56bright and CD56dim NK cell subsets for healthy volunteers, systemic inflammatory response syndrome (SIRS), and sepsis patients. Black line: isotype control; colored line: anti-TLR4. (B) Surface TLR4 expression shown as median and interquartile range in all three studied groups. (C) Representative flow cytometry histogram of intracellular expression of TLR4 for the same samples represented in (A). Black line: isotype control; colored line: anti-TLR4. (D) Intracellular TLR4 expression shown as median and interquartile range in all three groups. *P < 0.05; **P < 0.01; ***P < 0.001 for comparison with healthy donors. #P < 0.05 for SIRS versus sepsis patients. MFI, mean fluorescence intensity.