Figure 2From: Metformin overdose causes platelet mitochondrial dysfunction in humansEffects of pH on human platelet oxygen consumption. Platelets from healthy donors were incubated in plasma with saline (white bar) or metformin diluted in saline (16,600 mg/L; grey bar), lactic acid (to mimic metformin-induced lactic acidosis; dark grey bar) or metformin diluted in saline (16600 mg/L) plus sodium bicarbonate (to correct metabolic acidosis; black bar). After 72 hours, (a) plasma pH (P < 0.001; one-way ANOVA), (b) lactate (P < 0.001; one-way ANOVA) and (c) bicarbonate (P < 0.001; one-way ANOVA) concentrations and (d) platelet oxygen consumption (P < 0.001; ANOVA on ranks) (VO2) were measured. Data are mean and SD, from four experiments. * P < 0.05 versus saline (Holm-Sidak method). ANOVA, analysis of variance; SD, standard deviation.Back to article page