Figure 6
Plasma and H 2 S. Panel A, residual H2S concentration ([H2S]r), two minutes after addition of 100 μM H2S in the plasma of control (open circles) and vitamin B12-treated (closed circles) rats. [H2S]r is expressed in percentage of the concentration of 100 μM present in a control PBS solution analyzed at the same time as the plasma. Within two minutes, H2S concentrations decreased by more than 90% in control and vitamin B12-treated rats respectively, with a significant difference between the two groups (*P < 0.05). Panel B, absorbance spectra of H2S added to sham rat whole blood at 50, 100 and 150 μM and measured after two minutes. In the inset, the percentage of residual [H2S] ([H2S]r), corresponding to the absorbance at 670 nm, is shown for each initial concentration. Depending of the initial [H2S] ([H2S]i), exogenous H2S concentrations decreased between 92 and 75%.