Figure 4From: Bench-to-bedside review: Clinical experience with the endotoxin activity assayAssay contamination study. Endotoxin activity (EA) assay buffer (1 ml) was spiked with either 230 pg (n = 3) or 1,000 pg (n = 3) of E. coli 055:B5 lipopolysaccharide (LPS) (3.58 EU/ng). Different control patients were used for each dose of LPS. Average EAA values are depicted in blue, error of measurement (1 standard deviation from mean) depicted in red At 1,000 pg per assay the endotoxin activity was statistically higher than the control (*P < 0.05, paired Student t-test) but still below the normal cutoff of 0.4.Back to article page