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Figure 1 | Critical Care

Figure 1

From: Bench-to-bedside review: Clinical experience with the endotoxin activity assay

Figure 1

Reaction mechanism for endotoxin activity assay utilizing anti-lipopolysaccharide monoclonal antibody and autologous whole blood. Complement opsonised IgM-lipopolysaccharide (LPS) complexes interact with CR1 and CR3 complement receptors on neutrophils, resulting in a priming of the NADPH oxidase complex. The neutrophil respiratory burst is evoked using zymosan and measured by chemiluminescent emission from luminol. The magnitude of respiratory burst is proportional to the logarithmic concentration of LPS in the sample.

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Critical Care

ISSN: 1364-8535