Enhanced neutrophil recruitment into the lung and liver after rhDNase treatment. (A, B) Neutrophil counts in the lung and liver of mice 6 (n = 5), 24 (n = 7), and 48 (n = 7) hours after CLP treated with either 5 mg/kg rhDNase (CLP + rhDNase) or 100 µl PBS (CLP) 1, 4, 7, 10, 21, 24, and 27 hours after CLP. **P < 0.01, ***P < 0.001 versus adjacent time point; #P < 0.05; ###P < 0.001 versus CLP. Chloracetatesterase staining of paraffin sections from the lung and liver of mice 24 hours after CLP treated with either 5 mg/kg rhDNase (CLP + rhDNase) or 100 µl PBS (CLP). Representative sections of the liver (C) and lung (D) are depicted. Scale bar indicated 100 μm. CLP, cecal ligation and puncture; DNase, desoxyribonuclease; NETs, neutrophil extracellular traps; PBS, phosphate-buffered saline; rh, recombinant human.