Increased cf-DNA/NETs release and DNase concentration in peripheral blood as well as continuous capability and capacity of NETs release by PMN after CLP. cf-DNA/NETs (A) and DNase (B) levels in serum of mice were determined 6, 24, and 48 hours after CLP or sham operation, that is laparotomy without cecum perforation. Naive mice served as controls. (C) Additionally, cf-DNA/NETs were quantified in the supernatants of freshly isolated PMN from naive, sham and CLP mice, respectively, and further stimulation with 0, 30, 50, and 100 nM PMA for three hours PMA **P < 0.01, ***P < 0.001 versus adjacent time point within one group; #P < 0.05, ##P < 0.01, ###P < 0.001 versus sham; $P < 0.05, $$$P < 0.001 CLP versus naïve. Cf, circulating freely; CLP, cecal ligation and puncture; DNase, desoxyribonuclease; n.d.: not detected; PMA, phorbol-12-myristate-13-acetate; PMN, neutrophils.