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Figure 3 | Critical Care

Figure 3

From: Free hemoglobin concentration in severe sepsis: methods of measurement and prediction of outcome

Figure 3

Free hemoglobin measured in postoperative patients and patients with severe sepsis using four methods. Free hemoglobin did not differ in postoperative and septic patients using three of the four methods. Therefore, free hemoglobin cannot serve as a biomarker for the diagnosis of severe sepsis in critical illness. Data are given in boxplots (median, quartile, minimum, maximum). For statistical evaluation the Mann-Whitney test was used. Methods used to measure free hemoglobin were ELISA and the spectrophotometric methods of Harboe [13], Noe [14] and Fairbanks [15].

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