Figure 2
Haemodynamic and respiratory changes. Averaged data over 30-minute periods for each of the six groups of sheep. The first event (either saline or LPS) was infused from 0 to 30 minutes and the second event (either saline, "fresh PRBC" or "stored PRBC") was infused from 90 to 150 minutes. The left column represents sheep receiving saline-infusion as a first event (that is, sham, saline-fresh and saline-stored groups), and the right column represents sheep receiving LPS-infusion as a first event (that is, LPS-control, LPS-fresh and LPS-stored groups). Dashed lines at O2sat = 90% and PaO2 = 125 mmHg (FiO2 was 40%, therefore PaO2/FiO2 = 300) represent clinical cut-offs for hypoxaemia. LPS-stored sheep developed lower MAP, CO, PaO2, and O2 sat as well as higher PAP relative to sham sheep. Also, saline-stored sheep displayed increased PAP relative to sham sheep. # P < 0.05 vs. sham group using a two-way ANOVA with Bonferroni's multiple comparisons adjustment. LPS-stored sheep developed lower static pulmonary compliance, CO, PaO2, and O2 sat as well as higher PAP relative to the LPS-control group. * P < 0.05 vs. LPS-control using a two-way ANOVA with Bonferroni's multiple comparisons adjustment. ANOVA, analysis of variance; CO, continuous cardiac output; FiO2, fraction of inspired oxygen; "fresh PRBC," pooled heat-inactivated supernatant from Day 1 human PRBC; LPS, lipopolysaccharide; MAP, mean arterial pressure; O2sat, oxygen saturation; PaO2, arterial partial pressure of oxygen; PAP, pulmonary artery pressure; "stored PRBC," pooled heat-inactivated supernatant from Day 42 human PRBC; TRALI, transfusion-related acute lung injury.