Figure 2
Effect of the rs2027432 and rs12048215 polymorphisms of the NLRP3 gene on IL-1β production. The whole-blood samples collected from trauma patients immediately after admission were mixed at a 1:1 ratio (vol/vol) with RPMI 1640 culture medium and incubated with 100 ng/ml bacterial lipopolysaccharide at 37°C for 4 hours. The levels of IL-1β in the supernatants were assayed by performing a sandwich ELISA. The data are presented as means ± SD. One-way analysis of covariance (ANCOVA) was used to assess statistical significance. *Dominant effect (variant homozygotes + heterozygotes vs wild homozygotes) assessed by ANCOVA: *1P = 0.003, *2P = 0.018. #Recessive effect (variant homozygotes vs heterozygotes + wild-type homozygotes) assessed by ANCOVA: #1P < 0.0001, #2P = 0.00016.