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Figure 4 | Critical Care

Figure 4

From: Sepsis induces albuminuria and alterations in the glomerular filtration barrier: a morphofunctional study in the rat

Figure 4

Sepsis was associated with reduced expression and changes in chemical structure of glomerular sialic acids. Representative light microphotograph of lectin histochemistry at 7 hours in sham-operated and CLP-treated rats. Panels A-D: Sialic acid linked α-2,6 to Gal/NAcGal was decreased during sepsis. SNA reactivity (detected by blue staining) was decreased in renal corpuscles of CLP (panels B and D) with respect to sham-operated rats (panels A and C). Panels E-N: Sialic acid linked to D-Gal(β-1,3)-D-GalNAc was decreased during sepsis. No direct reactivity to PNA was observable in either sham-operated (panels E and G) or septic rats (panels F and H). After neuraminidase digestion, PNA reactivity appeared (in brown) but with less intensity in CLP (panels L and N) than in sham-operated rats (panels I and M). Panels O-R: Sialic acids containing C7 and/or C8 and/or C9-O-acetyl groups in the side chain were increased after sepsis. After mild oxidation-neuraminidase treatments, PNA reactivity (in brown/yellow) appeared more intense in CLP than in sham-operated renal corpuscles. CLP, cecal ligation and puncture; PNA, peanut agglutinin (Arachis hypogaea); SNA, Sambucus nigra agglutinin.

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