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Figure 1 | Critical Care

Figure 1

From: The synthetic Tie2 agonist peptide vasculotide protects against vascular leakage and reduces mortality in murine abdominal sepsis

Figure 1

Vasculotide (VT) prevents capillary leakage and neutrophil transmigration in vivo. Mice were pretreated with VT (200 ng intraperitoneally) or phosphate-buffered saline (PBS) at 16 hours and 1 hour prior to cecal ligation and puncture (CLP). Sham animals were pretreated with PBS at 16 hours and 1 hour prior to surgery. Immediately after surgery, Evans blue dye was injected intravenously. (A) Evans blue dye content in peritoneal lavage (PL) fluid at 18 hours after CLP or sham operation. (B) White blood cell (WBC) counts and (C, D) differential cell counts from PL samples at 18 hours after CLP or sham operation. Data are expressed as mean ± standard error of the mean (n = 7 to 10 mice per group). *P < 0.05; **P < 0.01; **P < 0.001. n.s., not significant; OD, optical density; PMN, polymorphonuclear neutrophil.

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