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Figure 6 | Critical Care

Figure 6

From: mRNA-based approach to monitor recombinant gamma-interferon restoration of LPS-induced endotoxin tolerance

Figure 6

Gene expression restoration after incubation with recombinant IFN-γ in whole blood of septic shock patients. Gene expressions of (a) TNF-α, (b) IL-10, (c) HLA-DRA, (d) CIITA, (e) IRAK-M and (f) LY64 were quantified by quantitative RT-PCR in EDTA whole blood from eight septic shock patients sampled within three days after the onset of shock (black columns) and eight healthy volunteers (open columns), stimulated or not (control) with lipopolysaccharide (LPS; 100 ng/ml) ± rIFN-γ1b (100 ng/ml) for one night. Data are presented as mean ± standard deviation. Comparison between healthy volunteers and septic shock patients treated with LPS was performed using the Mann-Whitney U test (* P < 0.05) whereas evaluation of rIFN-y effect was performed using the Wilcoxon signed rank test († P < 0.05). IFN, interferon.

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