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Figure 4 | Critical Care

Figure 4

From: Mild-stretch mechanical ventilation upregulates toll-like receptor 2 and sensitizes the lung to bacterial lipopeptide

Figure 4

Toll-like receptor 2 expression in A549 cells stretched with various inhibitors. IL-8 mRNA levels were measured as a positive control of cell activation by cyclic stretch. mRNA levels were normalized with the expression of GAPDH mRNA. All values are reported as the fold increase compared with control static cells normalized to 1. SB203580 significantly reduced the toll-like receptor (TLR)2 mRNA level induced by stretch, whereas Sn50 had no effect. The opposite was observed for IL-8 expression induced by cyclic stretch. Results are expressed as the mean ± standard error of the mean of three distinct experiments. *P < 0.05 between stretched cells with or without SB 203580. SB, spontaneously breathing.

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Critical Care

ISSN: 1364-8535