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Figure 3 | Critical Care

Figure 3

From: Mild-stretch mechanical ventilation upregulates toll-like receptor 2 and sensitizes the lung to bacterial lipopeptide

Figure 3

Inflammatory mediator production by A549 cells stimulated with Pam 3 CSK 4 in static and cyclic stretch conditions. (a) IL-6 and IL-8 protein concentrations in conditioned supernatants from cells stretched for 24 hours or kept in static condition, and subsequently stimulated for 8 hours with bacterial lipopeptide (BLP). Cyclic stretch increased the inflammatory response of A549 cells in response to BLP. (b) IL-6 and IL-8 mRNA levels in human type II-like A549 alveolar cells stretched for 24 hours or kept in static condition, and subsequently stimulated for 8 hours with BLP. mRNA levels were normalized to the expression of GAPDH mRNA. All values are reported as the fold increase compared with control static cells normalized to 1. For both inflammatory markers, a clear synergistic effect between cyclic stretch and BLP is observed. Results are expressed as the mean ± standard deviation of four distinct experiments. *P < 0.05 between static and stretched cells stimulated with BLP.

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Critical Care

ISSN: 1364-8535