Skip to main content
Figure 5 | Critical Care

Figure 5

From: Injurious mechanical ventilation affects neuronal activation in ventilated rats

Figure 5

Brain activation evidenced by c-fos immunotreactivity in central amygdala. On the top (left): Coronal section diagram encompassing the area of interest. On the bottom: Representative images of the central amygdala from each experimental group after cresyl violet staining (left,a, c,e,g, 40X) and c-fos immunohistochemistry (right, 100X and 400X). Brown dots represent c-fos staining. Black arrows indicate some examples of c-fos positive cells. MV significantly increased c-fos immunoreactive cells in the CeA independently of the Vt level (f, h ). Few c-fos positive cells were found in CeA of spontaneous breathing (d) and basal (b) animals. Data are presented as mean ± SE. *P < 0.05 respect to unventilated basal animals. Abbreviations: No MV, unventilated animals; LVt, low tidal volume; HVt, high tidal volume; Spont, spontaneous breathing; CeA, central amygdala.

Back to article page