ELISA's to measure FSAP complexes with C1-inhibitor and α
-antiplasmin. To measure complexes of FSAP with AP, AAP-20 recognizing AP was used as catching antibody and biotinylated anti-FSAP-4 was used for detection (A). To measure complexes of FSAP with C1-inh, KOK12, a monoclonal antibody recognizing complexed C1-inh was used as a catching antibody. As detection biotinylated anti-FSAP-4 was used (B). Complexes were measured in r-plasma and r-plasma incubated with apoptotic cells. Results are given as mean ± SEM, (n = 3).