Volume 4 Supplement 1

20th International Symposium on Intensive Care and Emergency Medicine

Open Access

The role of selected biochemical values in graft quality prediction in brain death organ donors

  • V Cerny1,
  • P Zivny1,
  • R Parizkova1 and
  • P Dostal1
Critical Care20004(Suppl 1):P208

DOI: 10.1186/cc927

Published: 21 March 2000

Full text

Introduction

The aim of the study was to evaluate selected biochemical values in brain death kidney donors and to compare these results with number of rejection episodes in recipients.

Methods

After institutional approval 35 patients were prospectively studied. According to insult leading to brain injury, patients after brain death diagnosis (angiography) were divided into the two groups: donors with traumatic intracranial hemorrhage (Group D1) and donors with non-traumatic intracranial hemorrhage (Group D2). Blood samples were drawn immediately after brain death diagnosis. Lymphocyte count, CD3, CD4, CD8, CD19, CD56/16, CD25, ALT, AST, immunoglobulins (IgG, IgM, IgA), malondialdehyde, erythrocyte glutathione levels (GSH) and erythrocyte glutathione-peroxidase activity (GPX), selenium, sIL-2R, IL-2, IL-6, IL-8 and TNF-α were measured. Results as mean (SD), *P<0,05.

Results

Selected results are presented in the Table.

Conclusions

Selenium levels correlated with graft prognosis, significant differences in selected values were found between trauma and non-trauma brain-stem death organ donors. High selenium levels in donors seem to be of value in prediction of later graft rejection.

Table

 

Group D1 (n=17)

Group D2 (n=18)

Recipients no rejection

8

7

Rejection treated by steroids

6

6

Rejection resistant to steroids

1

3

AST (μkat/L)

   1.74± 0.36*

  1.29± 0.21

ALT (μkat/L)

   0.79± 0.11*

  1.47± 0.40

GPX (mg/g Hb)

   44± 5.4

39.9± 4.4

GSH (mg/g Hb)

 2.84± 0.3

  3.99± 0.75

MDA (μmol/L)

   0.75± 0.08

  0.66± 0.09

Serum IL-6 (pg/mL)

125.69± 35.6

 146.9± 41.6

Selenium (μmol/L)

    0.585± 0.062

   0.761± 0.077

Authors’ Affiliations

(1)
Department of Anesthesiology and Intensive Care, Charles University, Faculty of Medicine

Copyright

© Current Science Ltd 2000

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